| Autor: |
Izadnia, Fariba, Wong, Catarina, Kocoshis, Samuel, Izadnia, F, Wong, C T, Kocoshis, S A |
| Předmět: |
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| Zdroj: |
Digestive Diseases & Sciences; Sep1998, Vol. 43 Issue 9, p2055-2060, 6p |
| Abstrakt: |
Saccharomyces boulardii (Sb), a nonpathogenicyeast, has been used to prevent recurrences ofClostridium difficile (C.diff)-associated diarrhea. Asingle report suggested that treatment withSaccharomyces cerevisiae (Sc), commonly called brewer's yeast(BY), facilitates treatment of persistent C.diffinfection. We conducted this experiment to determinewhether C.diff toxin A-induced colonic secretion in the rat is blunted by pretreatment with eitherSb or BY. We employed closed cecal pouches in two groupsof five adult male Sprague-Dawley rats fed with standardchow for five days prior to the experiment, another group whose water was supplemented with20 × 109 colony-forming units (CFU) ofSb per day for five days, and another group whose waterwas supplemented with 20 × 109 CFU ofSc per day for five days. Cecal pouches were infused for 3 hr with one ofthe following: (1) normal saline alone for a controlgroup, or (2) normal saline plus 5 μg of C.diff toxinA (for the other control group and for the two experimental groups). Water movement wasmeasured by a nonabsorbable marker technique. Sodiummovement and permeability to mannitol were alsomeasured. Prior to the infusion, cecal contents werequantitatively cultured. In the three animals whose ceca werecolonized with less than 106 CFU of eitheryeast per gram wet cecal content, toxin A-inducedsecretion could not be attenuated. In contrast, animalswhose ceca were colonized with more than106 CFU of either yeast per gram of wet cecalcontent showed significantly less secretion after toxinA application than those which were not fed yeast. S.cerevisiae reduced secretion by half (N = 5, P = 0.039 forwater, 0.044 for sodium) and Sb by 75% (N = 4, P = 0.015for water, 0.034 for sodium). Toxin-induced increases inpermeability to [3H]mannitol from systemic circulation to cecum could not be blunted byeither yeast. We conclude that rat ceca can be colonizedby either organism and that both organisms reduce C.difftoxin A-mediated secretion. We speculate that both organisms might have benefit in humanC.diff-associated enterocolitis. Further studies oftheir mechanisms of action as well as clinical trialsfor the prevention and treatment of human C.diffinfections should be pursued. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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