QTL detection by multi-parent linkage mapping in oil palm ( Elaeis guineensis Jacq.).

Autor: Billotte, N., Jourjon, M. F., Marseillac, N., Berger, A., Flori, A., Asmady, H., Adon, B., Singh, R., Nouy, B., Potier, F., Cheah, S. C., Rohde, W., Ritter, E., Courtois, B., Charrier, A., Mangin, B.
Předmět:
Zdroj: Theoretical & Applied Genetics; May2010, Vol. 120 Issue 8, p1673-1687, 15p, 2 Diagrams, 5 Charts
Abstrakt: A quantitative trait locus (QTL) analysis designed for a multi-parent population was carried out and tested in oil palm ( Elaeis guineensis Jacq.), which is a diploid cross-fertilising perennial species. A new extension of the MCQTL package was especially designed for crosses between heterozygous parents. The algorithm, which is now available for any allogamous species, was used to perform and compare two types of QTL search for small size families, within-family analysis and across-family analysis, using data from a 2 × 2 complete factorial mating experiment involving four parents from three selected gene pools. A consensus genetic map of the factorial design was produced using 251 microsatellite loci, the locus of the Sh major gene controlling fruit shell presence, and an AFLP marker of that gene. A set of 76 QTLs involved in 24 quantitative phenotypic traits was identified. A comparison of the QTL detection results showed that the across-family analysis proved to be efficient due to the interconnected families, but the family size issue is just partially solved. The identification of QTL markers for small progeny numbers and for marker-assisted selection strategies is discussed. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index