Effect of the interaction between fibronectin and VLA-4 on the proliferation of human B cells, especially a novel human B-cell line, OPM-3.

Autor: Yoshida, Hitoshi, Nishiura, Tetsuo, Karasuno, Takahiro, Matsumura, Itaru, Ishikawa, Jun, Yoshimura, Masafumi, Yokota, Takafumi, Okajima, Yu, Ogawa, Megumu, Kanakura, Yuzuru, Tomiyama, Yoshiaki, Matsuzawa, Yuji
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Zdroj: British Journal of Haematology; Dec98, Vol. 103 Issue 3, p804-812, 9p, 2 Diagrams, 2 Charts, 3 Graphs
Abstrakt: Very late antigen (VLA)-4 integrin has been suggested to play an important role in haemopoiesis. However, little is known concerning the roles of the fibronectin (FN)/VLA-4 interaction in the proliferation of human B cells. In this study we investigated the effect of immobilized FN on the proliferation of various B-cell lines, including a newly-established B-cell line, OPM-3, and human tonsillar B cells, that primarily express VLA-4 but not VLA-5. Immobilized FN significantly promoted the proliferation of OPM-3 cells and normal B cells via VLA-4. The cross-linking of β1 integrins of OPM-3 cells resulted in the phosphorylation of the focal adhesion kinase (FAK) associated 90 kD protein, an increase in FAK-associated kinase activity, and the phosphorylation of Raf-1. Furthermore, the MEK1 inhibitor, PD98059, inhibited the FN-promoted proliferation of OPM-3 cells. These results demonstrate that the FN/VLA-4 interaction transmits the growth signal(s) which may be mediated by Ras pathway in OPM-3 cells, and suggest that OPM-3 cells may be of great value in studying the roles of the FN/VLA-4 interaction in human B-cell growth. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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