| Autor: |
Han, Ming-yong, Zheng, Shu, Yu, Jin-ming, Peng, Jia-ping, Guo, Qi-sen, Wang, Jia-lin |
| Zdroj: |
Journal of Zhejiang University: Science A; Apr2004, Vol. 5 Issue 4, p472-476, 5p |
| Abstrakt: |
To study the effect of interleukin-18 gene transfection on the tumorigenesis of breast cancer cell line Bacp37, human breast cancer cell line Bcap37 were transfected with Lipofectamine and selected by G418. The biological expression of rhlL-18 was tested by RT-PCR and ELISA method; nude mice were injected with Bcap37 cell with or without the hlL-18 gene. The hIL-18 cDNA was successfully integrated into Bcap37 cell; 126.3±4.5 pg hIL-18 secreted by one million transduced cells in 24 hours. Nude mice injected with IL-18 gene engineered Bcap37 cell had no tumor growth. These findings indicated that human breast cancer cells were successfully modified by the gene of IL-18 cytokine; the IL-18 gene engineered Bcap37 cells secreted hIL-18 and lost their tumorigenicity. The Bcap37 cells transduced with IL-18 gene may be used as breast cancer vaccine. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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