| Autor: |
Yinping, Huang, Duyun, Ye, Ping, Wu, Yanjun, Huang, Li, Zhang, Xiaoyan, Zhou, Yunfeng, Huang, Ping, Yuan, Daijuan, Zhang, Jingyuan, Wan |
| Zdroj: |
Journal of Huazhong University of Science & Technology, Medical Sciences; Feb2005, Vol. 25 Issue 1, p5-7, 3p |
| Abstrakt: |
In order to investigate the expression of cyclooxygenase-2 (COX-2) in human lower segments of myometrium obtained from women in labor and those not in labor and identify the splicing variant of COX-2, reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of COX-2. The primers were designed and synthesized according to the sequence of rat COX-2 splice variant which was discovered firstly by us. Then the splicing variant of COX-2 in human myometrium from woman in labor was identified, cloned into vector and sequenced. The results showed that the expression of COX-2 mRNA was lower in human myometrium obtained from women who were not in labor than that in labor women and a new band of COX-2 was obtained in myometrium from labor woman. The fragment included an unspliced intron, which pitched between exons 7 and 8. It was suggested that COX-2 gene was not only expressed highly in human myometrium from woman in labor, but also produced splicing variant by alternative splicing. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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