Synthesis of N-acyl homoserine lactones and phenazines, some enzymatic activities, and fungicidal activity in the cells of Pseudomonas chlororaphis 449 with inactivated rpoS gene.

Autor: Lipasova, V., Atamova, E., Khmel, I.
Zdroj: Molecular Genetics, Microbiology & Virology; Mar2009, Vol. 24 Issue 1, p7-11, 5p
Abstrakt: The inactivation of the rpoS gene, which encodes the sigma S subunit of RNA polymerase of rhizospheric strain Pseudomonas chlororaphis 449 results in a drastic (5–8-fold) decrease in the synthesis of phenazine antibiotics, a decline in the acid and alkaline phosphatase activities (pH 2.5 and 8.8, respectively), and the antagonistic activity of this strain against phytopathogenic fungus Rhizoctonia solani. A mutation in the rpoS gene also causes a minor decrease of lipase and proteolytic activity in the supernatants of Pseudomonas chlororaphis 449 and has no substantial effect on the synthesis of three types of N-acyl homoserine lactones, the signal molecules of quarum sensing regulation, and the ability of bacteria to migrate over the medium surface (swarming). [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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