| Autor: |
Matysik, G., Wójciak-Kosior, M. |
| Zdroj: |
Chromatographia; Jan2005, Vol. 61 Issue 1/2, p89-92, 4p |
| Abstrakt: |
The relationship RM= f (log % S; S- modifier) of several flavonoids and their sugar derivatives was examined by using chromatographic systems chosen by preliminary experiments. The dependence for flavonoid aglycones was flat in the whole mobile phase concentration. Two types of the RM= f (log % S) plots, related to the size of sugar molecule, were found. For glycosides with smaller sugar moiety (arabinose or glucose) the plot was almost parallel, while plots of derivatives with larger sugar moiety (rutose) were convergent. After plotting the relationship RM=f (log % S), even if standards are not available, it is possible to qualify the examined compound as containing single or complex sugar moiety. For all mentioned above compounds the RM= f (log % S) values were given. Silica gel Si60 was used as polar adsorbent. Mobile phase composition was determined in preliminary experiments; it was composed of two solvents: methanol in ethyl acetate in increasing concentration of methanol: 5 – 20%. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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