| Autor: |
Nemes, K., Szúnyog, J., Klebovich, I. |
| Zdroj: |
Chromatographia; Jul1998, Vol. 48 Issue 1/2, p158-162, 5p |
| Abstrakt: |
A new highly sensitive high-performance liquid chromatographic (HPLC) procedure for determination of EGIS-9933 (a newly developed anxiolytic compound) in rat plasma is described. A gradient, elution method with UV detection at 270 nm has been developed using a mobile phase of a mixture of A: methanol:acetonitrile 1:9 and B:0.5% triethilamine in water, the pH of B was adjusted to 3 with phosphoric acid. Solid phase extraction (SPE) was used for the sample preparation. The calibration was linear in the 10–10000 ng mL−1 concentration range. The limit of quantification was 10 ng mL−1. The bioanalytical method was validated according to internationally accepted criteria for biological samples. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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