A tumor suppressor gene fhit prokaryotic expression and identification.

Autor: Yan, Sun, Wen-xin, Li, Zan, Huang, Da-he, Jiang
Zdroj: Wuhan University Journal of Natural Sciences; Jun2000, Vol. 5 Issue 2, p249-252, 4p
Abstrakt: The recombinant expression vector pGEMD- fhit which contains full encoding region of fhit gene was constructed. The recombinant was introduced into the BL21 (DE3) strain of E. coli and induced by 1 mmol/L IPTG to express a 29×103 polypeptide of fhit fusion protein. And the 29×103 protein was sensitive and specific in reaction with anti- fhit antibody in Western blot. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index