A pyruvate dehydrogenase-based amperometric biosensor for assessing pungency in onions ( Allium cepa L.).

Autor: Abayomi, Louise, Terry, Leon
Zdroj: Sensing & Instrumentation for Food Quality & Safety; Dec2007, Vol. 1 Issue 4, p183-187, 5p
Abstrakt: A disposable prototype electrochemical biosensor was constructed using pyruvate dehydrogenase immobilised on mediated Meldolas Blue electrodes to determine pungency in onions ( Allium cepa L.). The optimum operating potential was +50 mV vs. Ag/AgCl reference/counter electrode. The biosensor was able to differentiate between mild and pungent bulbs with pyruvate concentrations ranging between ≈4 and 8 mM in freshly extracted juices. Resolution was 0.5 mM and thus was comparable to the standard Schwimmer and Weston-based colorimetric assay currently employed by industry for determining pungency in macerated onion tissue. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index