| Autor: |
SOARES, CRISTINA M. DIAS, ALVES, RITA C., CASAL, SUSANA, OLIVEIRA, M. BEATRIZ P.P., FERNANDES, JOSé OLIVEIRA |
| Předmět: |
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| Zdroj: |
Journal of Food Science (John Wiley & Sons, Inc.); Apr2010, Vol. 75 Issue 3, pT57-T63, 7p, 1 Diagram, 5 Charts, 4 Graphs |
| Abstrakt: |
The present study describes the development and validation of a new method based on a matrix solid-phase dispersion (MSPD) sample preparation procedure followed by GC-MS for determination of acrylamide levels in coffee (ground coffee and brewed coffee) and coffee substitute samples. Samples were dispersed in C18 sorbent and the mixture was further packed into a preconditioned custom-made ISOLUTE bilayered SPE column (C18/Multimode; 1 g + 1 g). Acrylamide was subsequently eluted with water, and then derivatized with bromine and quantified by GC-MS in SIM mode. The MSPD/GC-MS method presented a LOD of 5 μg/kg and a LOQ of 10 μg/kg. Intra and interday precisions ranged from 2% to 4% and 4% to 10%, respectively. To evaluate the performance of the method, 11 samples of ground and brewed coffee and coffee substitutes were simultaneously analyzed by the developed method and also by a previously validated method based in a liquid-extraction (LE) procedure, and the results were compared showing a high correlation between them. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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