α-Thalassemia-Like Globin Gene Expression by Primitive Erythrocytes Derived from Human Embryonic Stem Cells.

Autor:	Honig, George R., Lu, Shi-Jiang, Feng, Qiang, Vida, Loyda N., Lee, Bao-Shiang, Lanza, Robert
Předmět:	EMBRYONIC stem cells HEMOLYTIC anemia HEMOGLOBINOPATHY HUMAN embryos HEMOGLOBIN polymorphisms BLOOD cells
Zdroj:	Hemoglobin; Apr2010, Vol. 34 Issue 2, p145-150, 6p
Abstrakt:	Under culture conditions that promote hematopoietic differentiation, human embryonic stem cells (huESC) give rise to primitive erythroid cells that closely resemble the nucleated erythrocytes of early-stage human embryos. The globin chain distribution of these cells is similar to that seen during the embryonic and fetal stages of development. Here we show that huESC-derived erythroid cells produce substantial quantities of homotetrameric hemoglobin (Hb) composed exclusively of γ-globin-containing subunits. The globin synthesis of these erythroid cells was also significantly unbalanced, with a substantial decrease of α-like globin chain synthesis in relation to that of their β-like globins, a pattern characteristically associated with α-thalassemia (α-thal). This pattern of unbalanced globin synthesis appears to be an inherent feature of human erythroid cells that synthesize predominantly embryonic-stage globins. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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