Intense physical exercise increases systemic 11beta-hydroxysteroid dehydrogenase type 1 activity in healthy adult subjects.

Autor:	Dovio, Andrea, Roveda, Eliana, Sciolla, Chiara, Montaruli, Angela, Raffaelli, Andrea, Saba, Alessandro, Calogiuri, Giovanna, De Francia, Silvia, Borrione, Paolo, Salvadori, Piero, Carandente, Franca, Angeli, Alberto
Předmět:	EXERCISE DEHYDROGENASES ENZYME-linked immunosorbent assay HYDROCORTISONE MASS spectrometry BIOCHEMISTRY COMPARATIVE studies CORTISONE HEALTH INTERLEUKINS PHENOMENOLOGY RESEARCH methodology MEDICAL cooperation OXIDOREDUCTASES PHYSICAL fitness RESEARCH EVALUATION research RANDOMIZED controlled trials RESISTANCE training
Zdroj:	European Journal of Applied Physiology; Mar2010, Vol. 108 Issue 4, p681-687, 7p, 2 Charts, 3 Graphs
Abstrakt:	Intense physical exercise activates the hypothalamic-pituitary-adrenocortical axis but little is known about changes in glucocorticoid sensitivity at the target cell level. No data are available on the acute effects of exercise on 11beta-hydroxysteroid dehydrogenase (11beta-HSD) type 1 activity, which generates biologically active cortisol from inactive cortisone and is expressed also in skeletal muscle. Fifteen healthy, trained males (age mean +/- SE 28 +/- 1) were assessed on three non-consecutive days: at rest, during an endurance and strength sessions. During each session, between 1000 and 1600 hours, 6-h urine and four salivary samples were collected. Urinary total tetrahydrocortisol (THF) + alloTHF, tetrahydrocortisone (THE), cortisol (F) and cortisone (E) were measured with HPLC-tandem mass spectrometry; urinary-unconjugated F and E were measured by HPLC-UV. Salivary cortisol and interleukin (IL)-6 were measured by RIA and ELISA, respectively. Both endurance and strength exercises caused an increase in (THF + alloTHF)/THE ratio (mean +/- SE 1.90 +/- 0.07 and 1.82 +/- 0.05 vs. 1.63 +/- 0.06, P < 0.01 and P = 0.03, respectively), consistent with increased systemic 11beta-HSD type 1 activity. No relationship was found with age, BMI, VO(2max) maximal power load or perceived exertion. No significant change was apparent in F/E ratio, an index of 11beta-HSD type 2 activity. No effect of exercise on salivary cortisol and IL-6 was observed, whereas a significant effect of sampling time was found. Intense physical exercise acutely increases systemic 11beta-HSD type 1 activity in humans. Such an increase may lead to higher cortisol concentration in target tissues, notably in skeletal muscle where it could contribute to limit exercise-induced muscle inflammatory response. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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