| Autor: |
Hatta, Masumitsu, Yamamoto, Natsuo, Miyazato, Akiko, Ishii, Naoto, Nakamura, Kiwamu, Inden, Ken, Aoyagi, Tetsuji, Kunishima, Hiroyuki, Hirakata, Yoichi, Suzuki, Kazuo, Kaku, Mitsuo, Kawakami, Kazuyoshi |
| Předmět: |
|
| Zdroj: |
FEMS Immunology & Medical Microbiology; Mar2010, Vol. 58 Issue 2, p182-192, 11p, 7 Graphs |
| Abstrakt: |
In this study, we elucidated the role of tumor necrosis factor (TNF)-α in the host defense to pulmonary infection with Streptococcus pneumoniae and defined the cellular source of this cytokine at an early stage of infection. Administration of anti-TNF-α monoclonal antibody (mAb) resulted in the reduced accumulation of neutrophils in bronchoalveolar lavage fluids (BALFs) and severe exacerbation of this infection. In a flow cytometric analysis, the intracellular expression of TNF-α was detected in Gr-1bright+ and Gr-1dull+ cells during the time intervals postinfection, and F4/80+ cells expressed intracellular TNF-α before Gr-1dull+ cells appeared. The Gr-1bright+ and Gr-1dull+ cells sorted from BALF cells at 24 h were identified as neutrophils and macrophage-like cells, respectively, and the Gr-1dull+ cells expressing CD11c, partially CD11b and a marginal level of F4/80 secreted TNF-α in in vitro cultures. Finally, deletion of Gr-1+ cells by administration of the specific mAb significantly reduced the concentrations of this cytokine in BALF at 6 and 12 h postinfection, but not the expression of TNF-α in F4/80+ cells. Thus, these results demonstrated that neutrophils, F4/80+ macrophages and Gr-1dull+ CD11c+ macrophage-like cells played an important role in the production of TNF-α in lungs at an early stage of infection with S. pneumoniae. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text