A multiplex PCR assay to distinguish among three sympatric marsupial taxa from Huon Peninsula, Papua New Guinea, using the mitochondrial control region.

Autor:	McGREEVY JR., T. J., DABEK, L., HUSBAND, T. P.
Předmět:	POLYMERASE chain reaction MARSUPIALS MAMMALS KANGAROOS MITOCHONDRIAL DNA
Zdroj:	Molecular Ecology Resources; Mar2010, Vol. 10 Issue 2, p397-400, 4p, 1 Chart, 1 Graph
Abstrakt:	Matschie’s tree kangaroo ( Dendrolagus matschiei) is an endangered arboreal macropodid marsupial that is difficult to locate in the wild. The Tree Kangaroo Conservation Program noninvasively collected D. matschiei faecal samples, but a high percentage of samples were misidentified and belonged to two terrestrial macropods, New Guinea pademelon ( Thylogale browni) and small dorcopsis ( Dorcopsulus vanheurni). We developed species-specific primers that produced a unique sized mitochondrial DNA control region gene product for each taxon and achieved an 89% correct assignment rate. The species-specific primers can increase the accuracy of D. matschiei density estimates and facilitate additional genetic analyses of wild D. matschiei. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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