| Autor: |
König, Edwin A., Köves, István, Răşinariu, Angela, Popp, Anghel R., Kusser, Wolfgang C., Soyonki, Ken, Kovács, Ágota, Glickman, Barry W., Jeney, Andrá, Marcsek, Zoltán L. |
| Předmět: |
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| Zdroj: |
Journal of Toxicology & Environmental Health: Part A; 2001, Vol. 62 Issue 5, p333-347, 15p |
| Abstrakt: |
Many molecular investigations of colorectal cancer (CRC) have suggested that the accumulation of specific mutations in proto-oncogenes and tumor suppressor genes regulating cell growth via signal transduction trigger the stagewise progression to malignancy. In this study, the frequency, location, and type of mutations of the K-ras proto-onco-gene exon 1 and p53 tumor suppressor gene exons 5-8 were analyzed in colorectal carcinomas of 65 patients from Central Europe, using polymerase chain reaction (PCR)-cold single-strand conformation polymorphism (SSCP) screening and direct sequencing. The incidence of K-ras activating mutations in these Central European samples was lower (25%) compared to that obtained in American and western European populations (40-50% at least), while the incidence of p53 inactivating mutations was similar (58%). These results suggest that some other genetically linked mechanisms may play a role in CRC development and progression, and hence K-ras and p53 mutations cannot be considered to be universal genetic markers for CRC. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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