Characterisation of verotoxin-producing Escherichia coli strains isolated from human patients in Hungary over a 7-year period.

Autor: Mag, T., Nógrády, N., Herpay, M., Tóth, I., Rozgonyi, F.
Předmět:
Zdroj: European Journal of Clinical Microbiology & Infectious Diseases; Feb2010, Vol. 29 Issue 2, p249-252, 4p, 1 Chart
Abstrakt: The purpose of this study was to characterise verotoxigenic Escherichia coli (VTEC) strains isolated in Hungary from 2000 to 2006. Altogether, 33 human VTEC strains were investigated to define the O:H antigens, verotoxin 1, 2 ( vtx1 and 2), intimin ( eae), enteroaggregative heat-stable toxin ( ast1), autoagglutinating adhesin ( saa) and enterohaemolysin ( ehlyA) genes and sensitivity to 11 antimicrobial agents. The strains belonged to 14 different O:H serotypes, among which O157:NM (non-motile) was the most prevalent (45%, 15/33). Patients infected with O157 more often presented bloody diarrhoea or haemorrhagic colitis (63%, 12/19) than those infected with non-O157 (46%, 6/14). Haemolytic uraemic syndrome evolved in two patients infected with O26:H11. The vtx1vtx2c toxin gene combination was found in 58% (11/19) and vtx2c alone in 31% (6/19) of the O157 strains. All of the O157 strains possessed γ1, while two O26 strains had the β1 intimin gene. Twenty strains (75%, 25/33) carried the ehlyA gene and five non-O157 strains had ast1. The majority of the strains (76%) were resistant to at least one antimicrobial agent, but none of them showed the extended-spectrum beta-lactamase (ESBL) phenotype. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index