| Autor: |
Spaska Angelova Stanilova |
| Předmět: |
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| Zdroj: |
Laboratory Medicine; Jan2010, Vol. 41 Issue 1, p47-50, 4p |
| Abstrakt: |
BACKGROUND: This study investigates the allele and genotype distribution of a single nucleotide polymorphism located at position +16974A/C in 3'-untranslated region (UTR) and a complex polymorphism (IL-12Bpro) within the promoter region of IL-12B gene in association with susceptibility to severe sepsis. METHODS: A total of 130 healthy volunteers and 43 patients, meeting the criteria for severe sepsis, were included. The polymorphism in 3'-untranslated region of the IL-12B was detected by restriction fragment length polymorphism assay. The allele-specific polymerase chain reaction was used for IL-12Bpro polymorphism detection. RESULTS: The results showed that healthy controls had significant elevation of IL-12Bpro-1 allele frequencies, compared to patients with sepsis (60% vs 44%; OR=1.895; 95% CI 1.157÷3.102; P=0.011). Homozygous genotype-11 was also more frequent in the healthy group than in the sepsis group (35% vs 16%; OR=0.358; 95% CI, 0.150÷0.855; P=0.02). CONCLUSIONS: We established that the IL-12Bpro-1 allele is associated with sepsis susceptibility, and genotype IL-12Bpro-11 could have a protective effect to the development of severe sepsis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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