Characteristics of two cancer cell lines derived from metastatic foci in liver and peritoneum of a patient with colon cancer.

Autor: Kotanagi, Hitoshi, Saito, Yuri, Yoshioka, Toshiaki, Koyama, Kenji, Kotanagi, H, Saito, Y, Yoshioka, T, Koyama, K
Předmět:
Zdroj: Journal of Gastroenterology; 1998, Vol. 33 Issue 6, p842-849, 8p
Abstrakt: Two cancer cell lines were established in vitro from a single patient with colon cancer; AKT-CC-K-LM cells from liver metastatic nodules and AKT-CC-K-PC cells from peritoneal dissemination nodules. The two cell lines were similar in doubling time, number of chromosomes, and chromosomal abnormalities. However, they differed in morphology in vitro, in the expression level of cell surface adhesion molecules (carcinoembryonic antigen; CEA, E-cadherin, sialyl Le(a), sialyl Le(x), and CD44v6), and in their metastatic properties. AKT-CC-K-LM cells grew in vitro as adherent clusters and AKT-CC-K-PC cells as adherent single cells. The expression levels of CEA, E-cadherin, sialyl Le(a), and sialyl Le(x) was significantly higher in AKT-CC-K-LM cells. The expression of CD44v6 was significantly higher in AKT-CC-K-PC cells. After the injection of AKT-CC-K-LM cells to the spleen or peritoneal cavity of severe combined immune deficiency mice, metastatic nodules were observed only in the liver. In contrast, the injection of AKT-CC-K-PC cells to the spleen or peritoneal cavity yielded metastatic nodules only in the peritoneal cavity. These cell lines may contribute to elucidating the relationship between cell surface adhesion molecules and the metastatic properties of cancer cells. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
Externí odkaz: