| Autor: |
Cánovas, A., Estany, J., Tor, M., Pena, R. N., Doran, O. |
| Předmět: |
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| Zdroj: |
Journal of Animal Science; Dec2009, Vol. 87 Issue 12, p3905-3914, 10p, 3 Charts, 5 Graphs |
| Abstrakt: |
The objectives of this study were 1) to determine whether selection toward less subcutaneous fat thickness at constant intramuscular fat content in pigs is related to tissue-specific changes in the expression of lipogenic enzymes acetyl-CoA carboxylase (ACC), stearoyl-CoA desaturase (SCD), and Δ6- desaturase (Δ6d); and 2) to investigate tissue specific distribution of the porcine ACC, SCD, and Δ6d. The study was conducted on 20 purebred Duroc barrows. Ten animals were from a group selected for decreased subcutaneous fat thickness at constant intramuscular fat content (experimental group). The other 10 animals were from the unselected (control) group. Distribution of ACC, SCD, and Md was investigated in semimembranosus muscle (SM), subcutaneous adipose tissue (SA), liver (L), kidney (K), heart (H), diaphragm (D), rectus capitis muscle (RCM), and abdominal fat (AF). The enzyme expression was studied in 10 animals in the case of SM and SA and in 4 animals in the case of other tissues. The following expression pattern was established for ACC: SM ≤H = K ≤D 0.1) in ACC and SCD protein expression between the control and experimental groups was observed in SM. Expression of Δ6d protein did not differ between the control and experimental groups for SA (P = 0.47) or SM (P = 0.31). There was a positive relationship between muscle SCD protein expression and intramuscular fat. content (r = 0.48, P < 0.05). Intramuscular fat content did not correlate with ACC or Δ6d protein expression (P = 0.23 and P = 0.80, respectively). We conclude that SCD might be an effective potential biomarker for intramuscular fat deposition. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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