| Autor: |
He, Yao, Sadahiro, Tomohito, Noh, Sang-Ik, Wang, Hong, Todo, Tsuyoshi, Chai, Ning-ning, Klein, Andrew S., Wu, Gordon D. |
| Předmět: |
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| Zdroj: |
Hepatology Research; Dec2009, Vol. 39 Issue 12, p1208-1218, 11p, 1 Color Photograph, 3 Black and White Photographs, 1 Chart, 2 Graphs |
| Abstrakt: |
Aims: Macrophages in the liver are well known for their functional heterogeneity. However, subpopulations of the hepatic macrophages are not well defined. Methods: Two subsets of hepatic macrophages isolated from rats via FACS with immunolabeling of ED2 (anti-CD163) antibody were studied for phenotypic and functional characteristics. Results: A subset showed an ED2high and autofluorescencehigh (ED2high/AFhigh) phenotype, exhibiting characteristics consistent with the description of the Kupffer cells (KC). A second subset, displaying an ED2dim/AFdim phenotype, was smaller in size, monocyte-like and weak in phagocytosis. Transmission electron microscopy demonstrated that both subsets are phagocytes. Quantitative RT-PCR revealed that in addition to expression of macrophage-related surface markers such as CD14, ED1 ( CD68), fucose receptor, and CD163, the ED2dim/ AFdim cells expressed mRNA encoding for myeloid lineage differentiation markers ERMP12 ( PECAM) and ERMP20 ( Ly-6C). These two subsets exhibited differential in gene expression of selected cytokines, extracellular matrix proteinases, and Toll-like receptor in normal livers, as well as significantly upregulated expression in cholestatic livers induced by bile duct ligation. Conclusion: The data suggest that the ED2high/AFhigh population of the liver cells represent the conventional Kupffer cells. The ED2dim/AFdim cells, however, are small hepatic resident macrophages characteristically different from the conventional Kupffer cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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