Evidence for different mechanisms of chloroquine resistance in 2 Plasmodium species that cause human malaria.
| Autor: | Nomura, Takashi, Rathore, Dharmendar, Fidock, David A., Su, Xin-zhuan, McCutchan, Thomas F., Wellems, Thomas E., Carlton, Jane M-R., Wootton, John C., Fryauff, David J., Collins, William E., Baird, J. Kevin, del Portillo, Hernando A., Nomura, T, Carlton, J M, Baird, J K, del Portillo, H A, Fryauff, D J, Rathore, D, Fidock, D A, Su, X |
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| Předmět: |
MALARIA
PLASMODIUM CHLOROQUINE GENETICS AMINO acids ANIMAL experimentation COMPARATIVE studies DOCUMENTATION DRUG resistance GENES GENETIC techniques RESEARCH methodology MEDICAL cooperation MICROBIOLOGICAL techniques MOLECULAR chaperones GENETIC mutation PARASITOLOGY PROTOZOA RESEARCH EVALUATION research |
| Zdroj: | Journal of Infectious Diseases; 6/1/2001, Vol. 183 Issue 11, p1653-1661, 9p, 2 Black and White Photographs, 1 Diagram |
| Abstrakt: | Chloroquine (CQ)-resistant Plasmodium vivax malaria was first reported 12 years ago, nearly 30 years after the recognition of CQ-resistant P. falciparum. Loss of CQ efficacy now poses a severe problem for the prevention and treatment of both diseases. Mutations in a digestive vacuole protein encoded by a 13-exon gene, pfcrt, were shown recently to have a central role in the CQ resistance (CQR) of P. falciparum. Whether mutations in pfcrt orthologues of other Plasmodium species are involved in CQR remains an open question. This report describes pfcrt homologues from P. vivax, P. knowlesi, P. berghei, and Dictyostelium discoideum. Synteny between the P. falciparum and P. vivax genes is demonstrated. However, a survey of patient isolates and monkey-adapted lines has shown no association between in vivo CQR and codon mutations in the P. vivax gene. This is evidence that the molecular events underlying P. vivax CQR differ from those in P. falciparum. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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