| Autor: |
Kovalyova, M. A., Kovalyov, L. I., Toropygin, I. Yu., Shigeev, S. V., Ivanov, A. V., Shishkin, S. S. |
| Předmět: |
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| Zdroj: |
Biochemistry (00062979); Nov2009, Vol. 74 Issue 11, p1239-1252, 14p, 2 Black and White Photographs, 1 Diagram, 3 Charts, 3 Graphs |
| Abstrakt: |
Proteins from bioptates and autoptates of human skeletal muscle m. vastus lateralis were separated by O’Farrell two-dimensional gel electrophoresis (2DE). MALDI-TOF MS and MS/MS enabled identification of 89 protein spots as expression products of 55 genes. A modification of the O’Farrell’s method including non-equilibrium electrophoresis in a pH gradient allowed detection — among major sarcomeric, mitochondrial, and cytosolic proteins — of several proteins, such as PDZ- and LIM domain-containing ones (p I > 8.70), fragments of known proteins, and a stable complex of heavy and light ferritin chains. The data underlie further studies of human skeletal muscle proteins in terms of molecular mechanisms of some physiological and pathological processes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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