| Autor: |
Picard, Chistophe, Frassati, Coralie, Basire, Agnes, Buhler, Stephan, Galicher, Vital, Ferrera, Virginie, Reviron, Denis, Zappitelli, Jean-Pierre, Bailly, Pascal, Chiaroni, Jacques |
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| Zdroj: |
Transfusion; Nov2009, Vol. 49 Issue 11 Part 1, p2412-2417, 6p, 3 Charts |
| Abstrakt: |
BACKGROUND: Anti-Fya has been implicated in hemolytic transfusion reactions. However, not all Fy(a−) patients develop anti-Fya after transfusion with 1 unit of blood [Fy(a+)]. This study was designed to identify HLA-DRB1 alleles associated with a predisposition to Fya immunization after blood transfusion. STUDY DESIGN AND METHODS: To identify HLA-DRB1 alleles prone to immunization after blood transfusion or pregnancy, HLA-DRB1 genotyping using polymerase chain reaction with sequence-specific oligonucleotide nonradioactive probe/sequence-specific priming methods was performed on blood samples from 67 immunized patients and 200 unrelated controls from the same southern European population in a case-control retrospective study. RESULTS: Ninety-six percent of patients with anti-Fya had at least one HLA-DRB1* 04 or HLA-DRB1* 15 allele compared to 34% of controls (pc < 0.001). Furthermore HLA-DRB1* 04 and HLA-DRB1* 1501 frequencies were significantly increased in Fya-immunized patients (35% vs. 12%, pc < 0.001; and 30% vs. 19%, pc < 0.001, respectively). Among HLA-DRB1* 04 allelic subtypes, DRB1* 0401 and DRB1* 0403 alleles were more strongly correlated with Fya immunization (51% vs. 24% and 19% vs. 9%; pc < 0.001, respectively). CONCLUSIONS: This study indicated that HLA-DRB1* 04 and DRB1* 1501 are overrepresented in Fya-immunized patients. The correlation between these alleles and Fya immunization could be due to a particular presentation of the Fya peptide in HLA-DRB1 molecules. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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