Clinical significance of anti-HEV IgA in diagnosis of acute genotype 4 hepatitis E virus infection negative for anti-HEV IgM.

Autor:	Shujun Zhang, Deying Tian, Zhengang Zhang, Junhui Xiong, Quan Yuan, Shengxiang Ge, Jun Zhang, Ningshao Xia, Zhang, Shujun, Tian, Deying, Zhang, Zhengang, Xiong, Junhui, Yuan, Quan, Ge, Shengxiang, Zhang, Jun, Xia, Ningshao
Předmět:	HEPATITIS E PHYSICIAN practice patterns RNA VIRAL hepatitis LIVER diseases DIAGNOSIS COMPARATIVE studies ENZYME-linked immunosorbent assay HEPATITIS viruses IMMUNOGLOBULINS RESEARCH methodology MEDICAL cooperation RESEARCH VIRAL antibodies VIRAL antigens EVALUATION research GENOTYPES
Zdroj:	Digestive Diseases & Sciences; Nov2009, Vol. 54 Issue 11, p2512-2518, 7p, 4 Charts
Abstrakt:	Anti-HEV IgM is a diagnostic for recent or ongoing HEV infection. However, some patients with acute hepatitis E (AHE) negative for anti-HEV IgM in acute period were often observed in clinical practice. In this study, we constructed the anti-HEV IgA indirect ELISA assay to evaluate the significance of anti-HEV IgA. The specificity of anti-HEV IgA was 99.6%. Among 245 AHE patients, 84 samples from 84 patients were positive for HEV RNA. The positive rate of anti-HEV IgA, anti-HEV IgM and anti-HEV IgG in 84 samples positive for HEV RNA was 96.3, 97.6, and 88.1%, respectively, and no sample was negative for anti-HEV IgA and anti-HEV IgM simultaneously. Among 245 AHE patients, we found nine samples collected from nine patients in acute period were negative for anti-HEV IgM but positive for anti-HEV IgA and two samples were positive for HEV RNA. Detection of anti-HEV IgA can be a useful supplement for diagnosis of acute HEV infection especially in patients negative for anti-HEV IgM. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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