| Autor: |
King, Leslie B., Tolosa, Eva, Lenczowski, Joi M., Lu, Frank, Lind, Evan F., Hunziker, Rosemarie, Petrie, Howard T., Ashwell, Jonathan D. |
| Zdroj: |
International Immunology; Aug1999, Vol. 11 Issue 8, p1203-1216, 14p |
| Abstrakt: |
While Jun/Fos-containing transcription factors are known to be necessary for many TCR-mediated events in mature T cells, relatively little is known about their roles in thymocyte development. We have generated transgenic mice that express a trans-dominant-negative mutant of c-Jun (TAM-67) specifically in thymocytes. Expression of TAM-67 inhibited the up-regulation of AP-1-responsive genes such as c-jun and IL-2 in stimulated thymocytes from transgenic mice. In addition, altered thymocyte development in TAM-67-expressing mice was revealed by a decrease in thymic cellularity (~50%) which could be accounted for primarily by a reduction in the number of CD4+CD8+ thymocytes, a large percentage of which retained CD25. The decrease in the number of CD4+CD8+ thymocytes did not appear to be due to an enhanced rate of apoptosis but rather to a decrease in the number of CD4–CD8–CD25– cells in the S + G2/M stages of the cell cycle. These results indicate that Jun/Fos-containing transcription factors promote the proliferative burst that accompanies the transition from the CD4–CD8– to the CD4+CD8+ stage of thymocyte development. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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