Expression of the Arabidopsis thaliana Histone Gene AtHTA1 Enhances Rice Transformation Efficiency.

Autor: Ye Zheng, Xiao-Wei He, Ying-Hui Ying, Jiang-Feng Lu, Gelvin, Stanton B., Hui-Xia Shou
Předmět:
Zdroj: Molecular Plant (Oxford University Press / USA); Jul2009, Vol. 2 Issue 4, p832-837, 6p, 1 Diagram, 2 Charts, 2 Graphs
Abstrakt: We expressed the Arabidopsis thaliana histone AtHTA1 in rice under the control of the maize ubiquitin promoter. Transformation efficiencies of rice plants that constitutively expressed AtHTA1 were 28–44% higher than calli containing an empty vector control. Furthermore, co-infection of rice calli with a vector containing AtHTA1 and another vector with the target gene increased transformation by 27–50%. Thus, expression of AtHTA1 either transiently or in stably transformed cells improved rice transformation efficiency. [ABSTRACT FROM PUBLISHER]
Databáze: Complementary Index