Evaluation of daptomycin susceptibility testing by Etest and the effect of different batches of media.

Autor: Fuchs, Peter C., Barry, Arthur L., Brown, Steven D., Fuchs, P C, Barry, A L, Brown, S D
Zdroj: Journal of Antimicrobial Chemotherapy (JAC); Oct2001, Vol. 48 Issue 4, p557-561, 5p
Abstrakt: One hundred and ninety-five Gram-positive bacteria representing 17 species were tested for susceptibility to daptomycin by broth microdilution and Etest methods. The geometric mean daptomycin MIC was 0.46 mg/L by broth microdilution tests and 0.73 mg/L by Etest. The concentration of calcium in 12 different batches of agar varied from 4 to 36 mg/L. Daptomycin Etest MICs varied inversely with the calcium concentration. Etest daptomycin MICs for quality control strains were within proposed quality control range on media with >20 mg/L of calcium. Monitoring the calcium levels of agar media by testing appropriate quality control strains is important for daptomycin Etests. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index