| Autor: |
Li, Jianjun, Bauer, SebastianH.J., Månsson, Martin, Moxon, E. Richard, Richards, James C., Schweda, Elke K.H. |
| Zdroj: |
Glycobiology; Dec2001, Vol. 11 Issue 12, p1009-1015, 7p |
| Abstrakt: |
A survey of both typeable and nontypeable strainsof Haemophilus influenzae indicatedthat they contain glycine (Gly) in their lipopolysaccharide (LPS).Significant amounts (30–250 pmol Gly/µgLPS) were determined by high-performance anion-exchange chromatographyusing pulsed amperometric detection after treatment of the LPS withmild alkali. Oligosaccharides obtained from LPS after mild acidhydrolysis and gel filtration chromatography were investigated byelectrospray ionization mass spectrometry (ESI-MS) and capillaryelectrophoresis (CE) ESI-MS. In all cases, molecular ions correspondingto the major glycoforms were identified and were accompanied byions differing by 57 Da, thus indicating the presence of glycine. Theposition of glycine in these glycoforms was determined by CE-ESI-MS/MSanalyses. It was found that, depending on strain, glycine can substituteeach of the heptoses of the inner-core element, l-α-d-Hepp-(1→2)-[PEtn→6]-l-α-d-Hepp-(1→3)-l-α-d-Hepp-(1→5)-α-Kdo of H. influenzaeLPSas well as Kdo. In some strains, mixtures of monosubstitutedGly-containing glycoforms having different substitution patternswere identified. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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