| Autor: |
Ptock, Jan A., Rafatmehr, Nassim, Sinovcic, Dubravko, Schnider, Jonas, Sakai, Hiromi, Tsuchida, Eishun, Banic, Andrej, Erni, Dominique |
| Předmět: |
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| Zdroj: |
American Journal of Physiology: Heart & Circulatory Physiology; Sep2009, Vol. 297 Issue 3, pH905-H910, 6p, 2 Color Photographs, 6 Graphs |
| Abstrakt: |
Local hypoxia, as due to trauma, surgery, or arterial occlusive disease, may severely jeopardize the survival of the affected tissue and its woundhealing capacity. Initially developed to replace blood transfusions, artificial oxygen carriers have emerged as oxygen therapeutics in such condi tions. The aim of this study was to target primary wound healing and survival in critically ischemic skin by the systemic application of leftshifted liposomal hemoglobin vesicles (HbVs). This was tested in bilateral, cranially based dorsal skin flaps in mice treated with a HbV solution with an oxygen affinity that was increased to a P50 (partial oxygen tension at which the hemoglobin becomes 50% saturated with oxygen) of 9 mmHg. Twenty percent of the total blood volume of the HbV solution was injected immediately and 24 h after surgery. On the first postoperative day, oxygen saturation in the critically ischemic middle flap portions was increased from 23% (untreated control) to 39% in the HbVtreated animals (P < 0.05). Six days postoperatively, flap tissue survival was increased from 33% (control) to 57% (P < 0.01) and primary healing of the ischemic wound margins from 6.6 to 12.7 mm (P < 0.05) after HbV injection. In addition, higher capillary counts and endothelial nitric oxide synthase expression (both P < 0.01) were found in the immunostained flap tissue. We conclude that leftshifted HbVs may ameliorate the survival and primary wound healing in critically ischemic skin, possibly mediated by endothelial nitric oxide synthaseinduced neovascularization. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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