| Autor: |
Mayta, Holger, Hancock, Kathy, Gilman, Robert H., Zamudio, Roxana, Castillo, Janeth P., Levine, Min Z., Garcia, Hector H., Gonzalez, Armando E., Tsang, Victor C. W. |
| Předmět: |
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| Zdroj: |
Journal of Parasitology; Jun2009, Vol. 95 Issue 3, p591-597, 7p, 1 Color Photograph, 2 Black and White Photographs, 1 Diagram |
| Abstrakt: |
Taenia solium Linnaeus, 1758 is responsible for taeniasis and cysticercosis, which are 2 serious health problems, particularly in developing countries. The attempt to identify a 22.5kD possible protective oncospheral antigen by 2-dimensional gel-electrophoresis, micro-sequencing, and cDNA library screening produced a protein of 42kD that possesses a conserved domain similar to that of troponin T. Five variants that showed differences at the 5′ end were observed at the cDNA level. Hyper-immune rabbit sera developed against recombinant GST fused protein identified the protein exclusively on activated oncospheres. The 42kD protein was tested in an enzyme-linked immunoassay (ELISA) alone and then together with the Tso31 protein for the diagnosis of human cysticercosis. When both antigens were combined, the test was found to be 85% sensitive and 65% specific. The 42kD is a novel T. solium protein that is present exclusively on activated oncospheres of this parasite, with poor diagnostic activity against taeniasis or human cysticercosis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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