| Autor: |
Koch, Kaspar, van Berkel, Sander S., van de Wal, Marloes M. E. B., Nieuwland, Pieter J., van Hest, Jan C. M., Rutjes, Floris P. J. T. |
| Předmět: |
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| Zdroj: |
Journal of Applied Physics; May2009, Vol. 105 Issue 10, p102012-102017, 5p, 1 Color Photograph, 1 Diagram, 4 Graphs |
| Abstrakt: |
The thrombin generation test is one of the diagnostic tests currently in use as a universal method for measuring hemostatic disorders. We envisioned that conventional monitoring of thrombin generation could be miniaturized resulting in a time-saving, accurate, easy-to-operate, and cost-efficient test. For the translation of the conventional thrombin generation test to microfluidic devices, our focus was directed to parameters such as the detection limit, temperature, protein-surface interactions (i.e., hydrophilicity of microchannels), and mixing behavior. Scaling down to microchannels (e.g., capillaries) resulted in volume reduction and allowed us to study the effect of a microchannel surface (either hydrophilic or hydrophobic) on the thrombin activity. Finally, the use of a micromixer enabled us to perform efficient on-chip mixing, resulting in the successful measurement of a thrombin generation in a microfluidic device. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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