| Autor: |
Oliver F. Stumpp, Vikramaditya P. Bedi, Danica Wyatt, Diana Lac, Zakia Rahman, Kin F. Chan |
| Předmět: |
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| Zdroj: |
Journal of Biomedical Optics; Mar2009, Vol. 14 Issue 2, p024018-024018-8, 1p |
| Abstrakt: |
In vivowound healing response post nonablative fractional laser treatment is evaluated. Seven healthy subjects receive treatments with a Fraxel re:store™ laser system on the forearm with pulse energies ranging from 10 to 70 mJ. The treatment sites are imaged at 1-hincrements up to 40 husing confocal microscope z-stacks using 10-m-depth spacing. At least five individual microscopic treatment zones are imaged per subject, time point, and treatment energy. Images are analyzed for tissue structure and morphology to classify each lesion as healed or not healed, depending on epidermal re-epithelialization at each time point and treatment energy. Probit analysis is used to statistically determine the ED50and ED84probabilities for a positive dose response (healed lesion) as a function of treatment energy. Confocal observations reveal epidermal keratinocyte migration patterns confirmed with histological analysis using hematoxylin and eosin (HE) and lactate dehydrogenase (LDH) staining at 10 mJat 0, 7, 16, and 24-hpost-treatment. Results indicate that more time is required to conclude re-epithelialization with larger lesion sizes (all less than 500 m) corresponding to higher treatment energies. For the entire pulse energy range tested, epidermal re-epithelialization concludes between 10 to 22-hpost-treatment for ED50and 13 to 28 hfor ED84. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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