| Autor: |
Zahedi, Kamyar, Lentsch, Alex B., Okaya, Tomohisa, Barone, Sharon, Sakai, Nozomu, Witte, David P., Arend, Lois J., Alhonen, Leena, Jell, Jason, Jänne, Juhani, Porter, Carl W., Soleimani, Manoocher |
| Předmět: |
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| Zdroj: |
American Journal of Physiology: Gastrointestinal & Liver Physiology; Apr2009, Vol. 296, pG899-G909, 11p, 2 Charts, 9 Graphs |
| Abstrakt: |
Expression of spermine/spermidine-N1 -acetyltransferase (SSAT), the rate-limiting enzyme of polyamine backconversion cascade, increases after ischemia-reperfusion injuries (IRI). We hypothesized that SSATplays an important role in the mediation of IRI. To test our hypothesis, wild-type (SSAT-wt) and SSAT-deficient (SSAT-ko) mice were subjected to liver or kidney IRI by ligation of hepatic or renal arteries. The liver and kidney content of putrescine (Put), a downstream by-product Of SSAT activity, increased in SSAT-wt animals but not in SSAT-ko animals after IRI, indicating that polyamine backconversion is not functional in SSAT-deficient mice. When subjected to hepatic IRI, SSAT-ko mice were significantly protected against liver damage compared with SSAT-wt mice. Similarly, .SSAT-ko animals subjected to renal IRl showed significantly greater protection against damage to kidney tubules than SSAT-wt mice. These studies indicate that SSAT-deficient animals are protected against IRI and suggest that SSAT is an important mediator of the tissue damage in IRI. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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