Surface plasmon optical study of the interfacial phase transition of elastinlike polypeptide grafted on gold.

Autor:	Xu, Fei, Joon, Huang Min, Trabbic-Carlson, Kimberly, Chilkoti, Ashutosh, Knoll, Wolfgang
Předmět:	SURFACE chemistry SURFACE plasmon resonance POLYPEPTIDES INTERFACES (Physical sciences) FLUORESCENCE spectroscopy ELASTIN GOLD
Zdroj:	Biointerphases; Sep2008, Vol. 3 Issue 3, p66-74, 9p, 1 Diagram, 8 Graphs
Abstrakt:	The conformational changes in elastinlike polypeptides (ELPs) grafted to a solid/solution interface via different architectures were studied using surface plasmon resonance spectroscopy and surface plasmon field-enhanced fluorescence spectroscopy (SPFS). SPFS provides a simple and convenient optical method to study the influence of the grafting method and the graft density on the conformational changes in ELPs at the solid-solution interface as a function of environmental variables. A typical response of the ELP, consistent with its stimuli responsiveness, was a gradual collapse upon increasing the ionic strength; this effect was inversely correlated with the surface graft density of the ELP. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
Externí odkaz:	Zobrazit plný text záznamu Full text from SpringerLink