| Autor: |
Dowd, F. J., Li, L. S., Campbell, J. E., Cheung, P. H. |
| Předmět: |
|
| Zdroj: |
Critical Reviews in Oral Biology & Medicine; 1993, Vol. 4 Issue 3, p415-419, 5p, 3 Charts |
| Abstrakt: |
Parotid acini were isolated and tested to further establish the presence of ecto-ATPase in the intact cells. Inhibitors were used to determine if the inhibitor profile of the ATPase was similar to that of a Ca2+-ATPase from parotid membranes identified previously as an ecto-ATPase. The Ca2+-ATPase of intact cells was insensitive to oligomycin (10 µg/ml), N-ethylmaleimide (NEM) (0.1 mM), ruthenium red (0.1 mM), sodium azide (1 mM), and was inhibited approximately 22% by sodium orthovanadate (Na3VO4) (1 mM). This profile was similar to the Ca2+-ATPase of intact cells. Trifluoperazine (TFP) (0.1 mM) inhibited the enzyme in intact cells by approximately 32%. The nucleotide substrate specificity of the enzyme also reflected very closely the pattern seen in isolated membranes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
