Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation.

Autor: Faccioli, L. H., Medeiros, A. I., Malheiro, A., Pietro, R. C. L. R., Januario, A., Vargaftig, B. B.
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Zdroj: Mediators of Inflammation; Feb98, Vol. 7 Issue 1, p41-47, 7p, 3 Graphs
Abstrakt: SERUM and BALF (bronchoalveolar lavage fluid) IL-8 levels and serum levels were investigated in Tox ocara canis infected guinea-pigs and the role of IL-5 as a modulator of cytokine secretion was studied. Serum levels increased early in infected animals, exceeding control levels 4 h after infection, peaked between days 6 and 18, and continued to exceed control levels after 48 days of infection. Serum and BALF IL-8 levels showed the same profile as blood eosinophilia, increasing 6 days post-infection and peaking between days 18 and 24. Treatment of infected animals with anti-IL-5 Ab suppressed eosinophilia with a parallel increase in blood IL-8 levels, whereas no change was found in levels. To support our in vivo observation we carried out experim ents in vitro using guinea-pig LPSstimulated adherent peritoneal cells which release large amounts of IL-8 into the supernatants. When rIL-5 was added to LPS-stimulated cells, 65% inhibition of IL-8 release into the supernatants was observed. Pre-incubation of cells with anti-IL-5 Ab prevented the inhibition of IL-8 release into the supernatants induced by rIL-5. Our results demonstrate for the first time that TNF- alpha and IL-8 are released concomitant with or after IL-5 in the eosinophilic inflammation induced by T. canis . Moreover, in addition to showing that IL-5 is fundamental for the induction of blood eosinophilia, the present results suggest that this cytokine may play a new biological role by acting as modulator of IL-8 secretion. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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