PCR-Based Survey of Anaplasma phagocytophilumin Portuguese Ticks (Acari Ixodidae).

Autor: Ana S. Santos, M. Margarida Santos-Silva, Rita de Sousa, Fatima Bacellar, J. Stephen Dumler
Předmět:
Zdroj: Vector-Borne & Zoonotic Diseases; Feb2009, Vol. 9 Issue 1, p33-40, 8p
Abstrakt: A total of two-thousand and six ticks, collected from 2002 to 2006 in areas belonging to seven districts of Mainland Portugal and also in Madeira Island, were examined by polymerase chain reaction (PCR) for the presence of Anaplasma phagocytophilum. Active infections were detected exclusively in Ixodesspecies, including six questing I. ricinusnymphs from Madeira Island, one questing I. ventalloinymph from Setúbal District, and two I. ventalloiadults found parasitizing domestic cats in both Setúbal and Santarém District. These findings confirm prior observations and suggest the persistence of A. phagocytophilumon Madeira Island. Moreover, it adds I. ventalloiand domestic cats to the list of potential elements of the agent's enzootic cycles in Portugal. Molecular analysis of PCR amplicons suggests the existence of two A. phagocytophilumgenotypes in Portugal, one of which is identical or very similar to North American strains implicated in human disease. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index