| Autor: |
Jiang-Qiang, K., Wei, W., Li-Na, W., Xiao-Dong, Z., Ke-Di, C., Ping, Z. |
| Předmět: |
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| Zdroj: |
Journal of Applied Microbiology; Mar2009, Vol. 106 Issue 3, p941-951, 11p, 1 Black and White Photograph, 4 Diagrams, 5 Charts, 2 Graphs |
| Abstrakt: |
Aims: To investigate the effect of the yeast-conform variant of the Artemisia annua gene encoding for amorpha-4,11-diene synthase (ADS) on the production of amorpha-4,11-diene in a transformed yeast. Methods and Results: The ADS gene was mutated to the yeast-conform variant ADSm. The ADSm synthesis was performed based on step-by-step extension of a short region of the gene through a series of polymerase chain reactions (PCR). The artificial ADSm gene contained codons preferred by the yeast translation machinery. The sequence was then integrated into a yeast expression vector pYeDP60. The fusion construct was active and the transformed yeast cells produced higher level of amorpha-4,11-diene compared with the plant gene-transformed yeast cells. Conclusions: Strains transformed with the yeast-conform allele ( ADSm) were more efficient in terms of production of amorpha-4,11-diene than those transformed with the plant gene. Significance and Impact of the Study: We demonstrated that yeast-conform allele of foreign genes by serial PCR reactions can be a solution to low efficiency of heterologous gene expression in Saccharomyces cerevisiae cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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