Occurrence of theobromine synthase genes in purine alkaloid-free species of Camellia plants.

Autor:	Ishida, Mariko, Kitao, Naoko, Mizuno, Kouichi, Tanikawa, Natsu, Kato, Misako
Předmět:	CAFFEINE PURINES ALKALOIDS CAMELLIAS PLANT cells & tissues PLANT metabolites MICROBIAL metabolites ADENINE GENES
Zdroj:	Planta: An International Journal of Plant Biology; Nov2008, Vol. 229 Issue 3, p559-568, 10p, 2 Black and White Photographs, 4 Diagrams, 2 Charts
Abstrakt:	Caffeine (1,3,7-trimethylxanthine) and theobromine (3,7-dimethylxanthine) are purine alkaloids that are present in high concentrations in plants of some species of Camellia. However, most members of the genus Camellia contain no purine alkaloids. Tracer experiments using [8-14C]adenine and [8-14C]theobromine showed that the purine alkaloid pathway is not fully functional in leaves of purine alkaloid-free species. In five species of purine alkaloid-free Camellia plants, sufficient evidence was obtained to show the occurrence of genes that are homologous to caffeine synthase. Recombinant enzymes derived from purine alkaloid-free species showed only theobromine synthase activity. Unlike the caffeine synthase gene, these genes were expressed more strongly in mature tissue than in young tissue. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
Externí odkaz:	Zobrazit plný text záznamu Full text from SpringerLink