| Autor: |
Zheng, G., Yampara-Iquise, H., Jones, J. E., Andrew Carson, C. |
| Předmět: |
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| Zdroj: |
Journal of Applied Microbiology; Feb2009, Vol. 106 Issue 2, p634-641, 8p, 3 Charts, 1 Graph |
| Abstrakt: |
Aims: The focus of this study was to identify a bacterial 16S rRNA gene sequence, unique to microbiota in the human gut, for use in development of a dependable PCR assay to detect human faecal pollution in water. Methods and Results: Suppression subtractive hybridization (SSH) and bioinformatics were used to identify a genetic marker, within the 16S rRNA gene of Faecalibacterium, for the detection of human faeces. DNA sequencing analysis demonstrated that a majority (16) of 74 clones of the SSH library contained insertion sequences identified as Faecalibacterium 16S rRNA genes . Human faeces-specific sequences were derived and six PCR primer sets designed and tested against faecal DNA samples from human and nonhuman sources. One PCR primer set, HFB-F3 and HFB-R5, was exclusively associated with human faeces. These primers generated a human faeces-specific amplicon of 399 bp from 60·2% of human faecal samples and 100% of sewage samples. Conclusions: The subject Faecalibacterium marker is specific for sewage. Significance and Impact of the Study: This study represents the initial report of a Faecalibacterium marker for human faeces, which may prove useful for microbial source tracking. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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