| Autor: |
Yano, Yuristella, Cesar, Katia R., Araujo, Magali, Rodrigues Jr, Adilson C., Andrade, Lucia C., Magaldi, Antonio J. |
| Předmět: |
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| Zdroj: |
American Journal of Physiology: Renal Physiology; Jan2009, Vol. 296, pF54-F59, 6p, 8 Graphs |
| Abstrakt: |
It is well known that Glucagon (Gl) is released after a high protein diet and participates in water excretion by the kidney, principally after a protein meal. To study this effect in in vitro perfused inner medullary collecting ducts (IMCD), the osmotic water permeability (Pf;µm/s) at 37°C and pH 7.4 in normal rat IMCDs (n = 36) perfused with Ringer/HCO3 was determined. Gl (10-7) in absence of Vasopressin (AVP) enhanced the Pf from 4.38 ± 1.40 to 11.16 ± 1.44 p.m/s (P < 0.01). Adding 10-8, 10-7, and 10-6 M Gl, the Pf responded in a dose-dependent manner. The protein kinase A inhibitor H8 blocked the 01 effect. The specific Gl inhibitor, des-His1-[Glu9] glucagon (l0-7), blocked the Gl-stimulated Pf but not the AVP-stimulated Pf. There occurred a partial additional effect between Gl and AVP. The cAMP level was enhanced from the control 1.24 ± 0.39 to 59.70 ± 15.18 fm/mg prot after Gl l0-7 in an IMCD cell suspension. The immunoblotting studies indicated an increase in AQP2 protein abundance of 27% (cont 100.0 ± 3.9 vs. GI 127.53; P = 0.0035) in membrane fractions extracted from IMCD tubule suspension, incubated with 10-66 M Gl. Our data showed that 1) Gl increased water absorption in a dose- dependent manner; 2) the anti-GI blocked the action of Gl but not the action of AVP; 3) Gl stimulated the cAMP generation; 4) Gl increased the AQP2 water channel protein expression, leading us to conclude that Gl controls water absorption by utilizing a Gl receptor, rather than a AVP receptor, increasing the AQP2 protein expression. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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