| Autor: |
Chabanon, Aurélie, Desterke, Christophe, Rodenburger, Emilie, Clay, Denis, Guerton, Bernadette, Boutin, Laetitia, Bennaceur-Griscelli, Annelise, Pierre-Louis, Olivier, Uzan, Georges, Abecassis, Lucile, Bourgeade, Marie-Françoise, Lataillade, Jean-Jacques, Le Bousse-Kerdilèsa, Marie-Caroline |
| Předmět: |
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| Zdroj: |
Stem Cells; Dec2008, Vol. 26 Issue 12, p3150-3161, 12p, 1 Diagram, 6 Graphs |
| Abstrakt: |
biology. A balance between quiescence and proliferation of hematopoietic stem cells in interaction with the microenvironment is critical for sustaining long-term hematopoiesis and for protection against stress. We analyzed the molecular mechanisms by which stromal cell-derived factor-1 (SDF-1) exhibited a cell cycle-promoting effect and interacted with transforming growth factor-β (TGF-β), which has negative effects on cell cycle orchestration of human hematopoietic CD34+ progenitor cells. We demonstrated that a low concentration of SDF-1 modulated the expression of key cell cycle regulators such as cyclins, cyclin-dependent kinase inhibitors, and TGF-β target genes, confirming its cell cycle-promoting effect. We showed that a cross-talk between SDF-1- and TGF-β-related signaling pathways involving phosphatidylinositol 3-kinase (PI3K)/Akt phosphorylation participated in the control of CD34+ cell cycling. We demonstrated a pivotal role of two downstream effectors of the PI3K/Akt pathway, FoxO3a and mammalian target of rapamycin, as connectors in the SDF-1-/TGF-β-induced control of the cycling/quiescence switch and proposed a model integrating a dialogue between the two molecules in cell cycle progression. Our data shed new light on the signaling pathways involved in SDF-1 cell cycle-promoting activity and suggest that the balance between SDF-1- and TGF-β-activated pathways is critical for the regulation of hematopoietic progenitor cell cycle status. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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