| Autor: |
Tong, Shifei, Yao, Qing, Wan, Ying, Zhou, Jianzhi, Shu, Maoqin, Zhong, Li, Li, Yonghua, Zhang, Qian, YinDai, Jiajia, Song, Zhiyuan |
| Předmět: |
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| Zdroj: |
Cardiology; 2008, Vol. 112 Issue 2, p114-121, 8p, 5 Color Photographs, 3 Diagrams |
| Abstrakt: |
Objective: To study the functional properties of If channels and the changes in mechanical activity of mouse mesenchymal stem cells (mMSCs) transfected with mHCN4. Methods: mMSCs were purified by using CD11b-immunomagnetic microbeads and transfected with pMSCV-mHCN4-EGFP or pMSCV-EGFP. We examined the kinetic characteristics of the mHCN4 channel. The morphological changes of positively transfected mMSCs were investigated at the same time. Results: The If current recorded from the experimental group was sensitive to extracellular Cs+ (–44.5 ± 4.2 vs. –5.5 ± 1.0 pA/pF, p < 0.001). The half-maximal activation was –99.0 ± 5.8 mV. The time constant of activation was 451 ± 61 ms under –140 mV. The control cells did not show the current under the same conditions. The absolute values of half-maximal activation decreased in the presence of cAMP or cGMP in the experimental group (–78.6 ± 10.4 and –85.7 ± 8.6 vs. –99.0 ± 5.8 mV, respectively, p < 0.05). mMSCs transfected with pMSCV-mHCN4-EGFP could form spontaneous beating cells. Extracellular Cs+ decreased the beating rate significantly (196 ± 50 vs. 66 ± 23 bmp, p < 0.01). Conclusions: Functional If channels can be reconstructed in mMSCs infected with mHCN4. mMSCs modified by successful transfection with mHCN4 can differentiate so as to develop spontaneous mechanical activity. Copyright © 2008 S. Karger AG, Basel [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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