| Autor: |
Chernov, Konstantin G., Mechulam, Alain, Popova, Nadezhda V., Pastre, David, Nadezhdina, Elena S., Skabkina, Olga V., Shanina, Nina A., Vasiliev, Victor D., Tarrade, Anne, Melki, Judith, Joshi, Vandana, Baconnais, Sonia, Toma, Flavio, Ovchinnikov, Lev P., Curmi, Patrick A. |
| Předmět: |
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| Zdroj: |
BMC Biochemistry; 2008, Vol. 9, Special section p1-16, 16p, 4 Black and White Photographs, 1 Diagram, 5 Graphs |
| Abstrakt: |
Background: YB-1 is a major regulator of gene expression in eukaryotic cells. In addition to its role in transcription, YB-1 plays a key role in translation and stabilization of mRNAs. Results: We show here that YB-1 interacts with tubulin and microtubules and stimulates microtubule assembly in vitro. High resolution imaging via electron and atomic force microscopy revealed that microtubules assembled in the presence of YB-1 exhibited a normal single wall ultrastructure and indicated that YB-1 most probably coats the outer microtubule wall. Furthermore, we found that YB-1 also promotes the assembly of MAPs-tubulin and subtilisin-treated tubulin. Finally, we demonstrated that tubulin interferes with RNA:YB-1 complexes. Conclusion: These results suggest that YB-1 may regulate microtubule assembly in vivo and that its interaction with tubulin may contribute to the control of mRNA translation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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