Quantification of chicken anaemia virus by competitive polymerase chain reaction.

Autor:	Yamaguchi, S., Kaji, N., Munang'andu, H. M., Kojima, C., Mase, M., Tsukamoto, K.
Předmět:	CHICKEN diseases ANEMIA POLYMERASE chain reaction
Zdroj:	Avian Pathology; Aug2000, Vol. 29 Issue 4, p305-310, 6p, 3 Diagrams, 2 Graphs
Abstrakt:	A quantitative method for chicken anaemia virus (CAV) was developed using competitive polymerase chain reaction (PCR). Competitive template was constructed by deletion of 33 nucleotides from a wildtype DNA clone of CAV. Quantification of CAV DNA molecules by the competitive PCR was rapid and highly reproducible when compared with conventional infectivity titration methods. The ratios of the viral DNA molecules and infectivity titres in MDCC-MSB1 cells varied between 1.3 and 3.55 log[sub 10] among several isolates, suggesting the existence of different infection efficiencies to MDCC-MSB1 cells by isolates. The competitive PCR will be useful for studying CAV infection in vivo and/or in vitro. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
Externí odkaz:	Zobrazit plný text záznamu Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.