| Autor: |
Parshina, N. A., Pleteneva, T. V., Baikova, V. N., Narimanov, M. N., Tyulyandin, S. A. |
| Předmět: |
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| Zdroj: |
Pharmaceutical Chemistry Journal; May2008, Vol. 42 Issue 5, p288-290, 3p, 1 Diagram, 1 Chart, 3 Graphs |
| Abstrakt: |
A method for estimating the antitumor agent gemcitabine in plasma by isocratic HPLC was developed. Sample preparation consisted of precipitating proteins with a mixture of acetonitrile and methanol (9:1) with subsequent acidification of the extract with glacial acetic acid. Supernatants were then evaporated to dryness in a rotary vacuum evaporator at 42°C. The dry residue was dissolved in the mobile phase, which consisted of a mixture of acetate buffer pH 5.0 and acetonitrile (97.5:2.5). UV detection of gemcitabine was performed at 282 nm. The detection limit for this compound was 1 µg/ml. This method can be used to study the pharmacokinetic characteristics of the agent within the frameworks of the protocol “Studies of dose-dependent regimes of long-term infusions of gemcitabine in patients with local and disseminated pancreas cancer.” [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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