| Autor: |
Brown, Patricia A., Bodles-Brakhop, Angela M., Draghia-Akli, Ruxandra |
| Předmět: |
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| Zdroj: |
DNA & Cell Biology; Nov2008, Vol. 27 Issue 11, p629-635, 7p, 1 Diagram, 4 Graphs |
| Abstrakt: |
A gene therapy treatment with plasmid-based growth hormone–releasing hormone (GHRH) delivered by electroporation (EP) was investigated during heat stress; 32 primiparous cows received 2.5 mg of a GHRH-expressing myogenic plasmid (pSP-HV-GHRH), while 20 were designated as controls. Offspring of treated animals showed a reduction in mortality (47%; p < 0.02), and survival from birth to 260 days was dramatically improved (0% mortality vs. 21% in controls) along with an increase in weight gain ( p < 0.05). Milk production was increased compared to controls with an average yield gain of 421 kg/cow ( p = 0.028). Prolactin (PRL) levels were also significantly increased compared to controls ( p < 0.05). The second pregnancy rate was improved by GHRH treatment (53.3% vs. 30.8%). This study shows that the use of plasmid-mediated therapy delivered by EP can maintain health status during periods of heat stress, important for both animals and potentially humans in hot, challenging climates. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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