Immersion oil for high-resolution live-cell imaging at 37°C: optical and physical characteristics.

Autor: OOMEN, L. C. J. M., SACHER, R., BROCKS, H. H. J., ZWIER, J. M., BRAKENHOFF, G. J., JALINK, K.
Předmět:
Zdroj: Journal of Microscopy; Nov2008, Vol. 232 Issue 2, p353-361, 9p, 1 Color Photograph, 2 Charts, 4 Graphs
Abstrakt: The use of normal immersion oil, developed for 23°C, at 37°C greatly compromises both axial resolution and signal intensity. We developed and characterized an immersion oil for optimal performance in live-cell imaging at 37°C. We quantify the improvements in resolution and intensity obtained when using the new oil instead of its standard 23°C counterparts. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index