| Autor: |
Sobti, Ranbir C., Gupta, Lipsy, Thakur, H., Seth, Amlesh, Singh, Shrawan K. |
| Předmět: |
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| Zdroj: |
Indian Journal of Urology; Supplement2, Vol. 24, pS113-S114, 2p |
| Abstrakt: |
Introduction: Various biomedical imaging modalities are under investigation for real time tissue imaging of periprostatic nerves with the idea of improving upon the results of nerve sparing radical prostatectomy. We explored the use of Multiphoton microscopy for this purpose. The unique advantage of this technique is the acquisition of high resolution image without requiring any extrinsic labeling agent and almost no phototoxic effect on tissue. Methods: The prostate and cavernous nerves were surgically excised in male Sprague-Dawley rats. An Olympus BX61WI upright fluorescence microscope was utilized. A femtosecond pulsed Titanium/sapphire laser at 780nm wavelength was used to excite the tissue. Second Harmonic Generation (SHG) signals were collected at 390 (± 35 nm) and the autofluorescence was collected at 380-530nm. The images were then merged and pseudocolors imparted for better appreciation of details. The corresponding tissues were subjected to H&E for histologic confirmation. Results: High resolution images of prostate capsule, underlying acini and the individual cell outlining the glands, fat and the neural tissues were obtained. Histologic confirmation and correlation of the prostate gland, fat, cavernous nerve and major pelvic ganglion validated the findings of multiphoton microscopy. Conclusions: Real time imaging and microscopic resolution of rostate and periprostatic neural tissue using multiphoton microscopy is feasible without the need for any extrinsic labeling agents. Integration of this imaging modality with operative technique has the potential to improve the precision of nerve sparing prostatectomy. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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